Hotspot

ndhF

SSC · 113066-115312 · 2247 bp

Back to Pterocyclus

Pi 0.00371

MarkerSeek score 45.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 16
pi: 0.00371
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCCTGCCCACACTAGAAAAA	GGGGGTAGGATCCGGTAAAT	50.0	55.0	57.7	58.3	2957-2966 bp	0.75	66.2
ndhF_p2	TCCCTGCCCACACTAGAAAA	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.6	58.3	2958-2967 bp	0.75	64.0
ndhF_p3	CCCTGCCCACACTAGAAAAA	GGGTAGGATCCGGTAAATCG	50.0	55.0	57.7	57.3	2955-2964 bp	0.75	62.4
ndhF_p4	CCCTGCCCACACTAGAAAAA	TTGAAAGGGGGTAGGATCCG	50.0	55.0	57.7	58.8	2963-2972 bp	0.75	62.0
ndhF_p5	TCCCTGCCCACACTAGAAAA	GGGTAGGATCCGGTAAATCG	50.0	55.0	58.6	57.3	2956-2965 bp	0.75	60.2