Hotspot

ndhF-rpl32

SSC · 115313-116517 · 1205 bp

Back to Pterocyclus

Pi 0.00816

MarkerSeek score 68.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1205
variable_sites: 18
pi: 0.00816
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAAGAAGAAGTCCCGCTCC	GACAACGCTTTTAAGGCTGC	50.0	50.0	57.8	58.3	1351-1422 bp	1.0	79.4
ndhF-rpl32_p2	AAGAAGAAGTCCCGCTCCTA	GACAACGCTTTTAAGGCTGC	50.0	50.0	57.8	58.3	1349-1420 bp	1.0	79.0
ndhF-rpl32_p3	AAAAGAAGAAGTCCCGCTCC	AGGCTGCCCAATATCCTTTC	50.0	50.0	57.8	57.6	1338-1409 bp	1.0	78.9
ndhF-rpl32_p4	CCACATACGGCGAAGACTTT	GACAACGCTTTTAAGGCTGC	50.0	50.0	58.3	58.3	1385-1456 bp	1.0	78.6
ndhF-rpl32_p5	AAGAAGAAGTCCCGCTCCTA	AGGCTGCCCAATATCCTTTC	50.0	50.0	57.8	57.6	1336-1407 bp	1.0	78.6