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psbT-psbN

LSC · 76898-76983 · 86 bp

Back to Pterocyclus

Pi 0.0291

MarkerSeek score 66.20

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 86
variable_sites: 5
pi: 0.0291
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	GAGAACCGCCTAAAGTTCCA	AGAGATCCGTTCGAGGAACA	50.0	50.0	57.5	58.2	148-167 bp	1.0	77.5
psbT-psbN_p2	GAGAACCGCCTAAAGTTCCA	TCGAGGAACATGGGGACTAG	50.0	55.0	57.5	58.2	138-157 bp	1.0	77.2
psbT-psbN_p3	GAGAACCGCCTAAAGTTCCA	TTCGAGGAACATGGGGACTA	50.0	50.0	57.5	57.8	139-158 bp	1.0	77.0
psbT-psbN_p4	AACCGCCTAAAGTTCCAACT	AGAGATCCGTTCGAGGAACA	45.0	50.0	57.3	58.2	145-164 bp	1.0	76.1
psbT-psbN_p5	GAGAACCGCCTAAAGTTCCA	GGGTACGCCTTATATACCGC	50.0	55.0	57.5	57.7	193-212 bp	1.0	76.0