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rps12-clpP

LSC · 72401-72555 · 155 bp

Back to Pterocyclus

Pi 0.0162

MarkerSeek score 60.50

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 155
variable_sites: 5
pi: 0.0162
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	GTACATGTTCCTCGACGCTG	TGATCTTGTAGCGGTGGTTG	55.0	50.0	58.7	57.9	283-284 bp	1.0	75.1
rps12-clpP_p2	GTACATGTTCCTCGACGCTG	TGTTGATCTTGTAGCGGTGG	55.0	50.0	58.7	57.9	286-287 bp	1.0	75.1
rps12-clpP_p3	GTACATGTTCCTCGACGCTG	GTTGATCTTGTAGCGGTGGT	55.0	50.0	58.7	57.9	285-286 bp	1.0	75.1
rps12-clpP_p4	GTACATGTTCCTCGACGCTG	TATGTCAGCAACAGAAGCCC	55.0	50.0	58.7	57.9	319-320 bp	1.0	74.7
rps12-clpP_p5	AGTACATGTTCCTCGACGCT	TGATCTTGTAGCGGTGGTTG	50.0	50.0	58.8	57.9	284-285 bp	1.0	74.3