Hotspot

ndhC-trnV-UAC

LSC · 52989-54157 · 1169 bp

Back to Pterocyclus

Pi 0.0067
MarkerSeek score 60.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1169
variable_sites
15
pi
0.0067
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCAGGCCCTTTGTTAATCGG CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.9 58.1 1315-1322 bp 1.0 80.2
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.9 58.1 1297-1304 bp 1.0 80.1
ndhC-trnV-UAC_p3 TCAGGCCCTTTGTTAATCGG AACGCATGTTGGGTCTTTGA 50.0 45.0 57.9 58.3 1379-1386 bp 1.0 78.4
ndhC-trnV-UAC_p4 TCAGGCCCTTTGTTAATCGG ACGCATGTTGGGTCTTTGAA 50.0 45.0 57.9 58.3 1378-1385 bp 1.0 78.4
ndhC-trnV-UAC_p5 GGGGCTAAAACTCCGGAAAT ACGCATGTTGGGTCTTTGAA 50.0 45.0 57.9 58.3 1360-1367 bp 1.0 78.3