Hotspot

trnE-UUC-trnT-GGU

LSC · 32786-33640 · 855 bp

Back to Pterocyclus

Pi 0.00585

MarkerSeek score 65.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 855
variable_sites: 10
pi: 0.00585
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	950-2471 bp	1.0	80.4
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	915-2436 bp	1.0	79.7
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	947-2468 bp	1.0	79.2
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	TTGAACCGATGACTTACGCC	50.0	50.0	57.8	58.3	952-2473 bp	1.0	78.2
trnE-UUC-trnT-GGU_p5	TTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	55.0	50.0	57.4	58.0	985-2471 bp	1.0	77.3