Hotspot

petN-psbM

LSC · 30151-31534 · 1384 bp

Back to Pterocyclus

Pi 0.0207

MarkerSeek score 53.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1384
variable_sites: 31
pi: 0.0207
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTCCCTTTTTGACTCTGCG	TTTTCGTTTCGCCCTTCTCA	50.0	45.0	58.1	58.0	885-2292 bp	1.0	84.8
petN-psbM_p2	TGGCTGAGTCTTGAATTCCG	TTTTCGTTTCGCCCTTCTCA	50.0	45.0	57.9	58.0	1260-2677 bp	1.0	84.7
petN-psbM_p3	GGCTGAGTCTTGAATTCCGT	TTTTCGTTTCGCCCTTCTCA	50.0	45.0	57.9	58.0	1259-2676 bp	1.0	84.7
petN-psbM_p4	TGGCTGAGTCTTGAATTCCG	CTTTCCCTTTTCGTTTCGCC	50.0	50.0	57.9	58.0	1260-2684 bp	1.0	84.6
petN-psbM_p5	GGCTGAGTCTTGAATTCCGT	CTTTCCCTTTTCGTTTCGCC	50.0	50.0	57.9	58.0	1259-2683 bp	1.0	84.6