Hotspot

psbA-trnK-UUU

LSC · 1669-1882 · 214 bp

Back to Pterocyclus

Pi 0.0142

MarkerSeek score 61.70

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 214
variable_sites: 6
pi: 0.0142
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAAAGACGGTTTTCGGTGCT	CTTGGGGAGGGGTTTTTACC	45.0	55.0	57.8	58.1	380-385 bp	1.0	79.4
psbA-trnK-UUU_p2	GTAAAGACGGTTTTCGGTGC	CTTGGGGAGGGGTTTTTACC	50.0	55.0	57.7	58.1	381-386 bp	1.0	78.9
psbA-trnK-UUU_p3	TAAAGACGGTTTTCGGTGCT	GAAAGCCGTGTGCAATGAAA	45.0	45.0	57.8	57.9	413-418 bp	1.0	78.7
psbA-trnK-UUU_p4	TAAAGACGGTTTTCGGTGCT	CTAGTTCCGGGTTCGAATCC	45.0	55.0	57.8	57.8	328-333 bp	1.0	78.5
psbA-trnK-UUU_p5	GTAAAGACGGTTTTCGGTGC	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.7	57.9	414-419 bp	1.0	78.2