Hotspot

accD

LSC · 62337-63911 · 1575 bp

Back to Pterocarya

Pi 0.00147

MarkerSeek score 37.40

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1575
variable_sites: 9
pi: 0.00147
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TGGATCCTTAGGGTTGGTGT	GATCCGGGACTAGGGATTCT	50.0	55.0	57.9	57.7	2343-2398 bp	1.0	79.2
accD_p2	GGCTGCTTGTGAAGTATGGA	GATCCGGGACTAGGGATTCT	50.0	55.0	57.9	57.7	2620-2685 bp	1.0	78.8
accD_p3	CCCGTAGTTTCCGATCATGG	GATCCGGGACTAGGGATTCT	55.0	55.0	58.1	57.7	2301-2356 bp	1.0	78.6
accD_p4	ATTGTTGTGTTGGGTCCACA	GATCCGGGACTAGGGATTCT	45.0	55.0	57.8	57.7	2373-2428 bp	1.0	78.4
accD_p5	GATCATGGATCGAGCCACAT	GATCCGGGACTAGGGATTCT	50.0	55.0	57.5	57.7	2289-2344 bp	1.0	75.9