Hotspot

ndhF-rpl32

SSC · 118123-118956 · 834 bp

Back to Pterocarya

Pi 0.0054

MarkerSeek score 47.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 834
variable_sites: 14
pi: 0.0054
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CACATGCGGCGAAGATTTTT	GCTACCCAATACCCCTTCCT	45.0	55.0	58.0	58.5	1024-1036 bp	1.0	78.2
ndhF-rpl32_p2	AATCGGTCGTGTGAACCAAA	GCTACCCAATACCCCTTCCT	45.0	55.0	58.0	58.5	1415-1427 bp	1.0	77.6
ndhF-rpl32_p3	ATCGGTCGTGTGAACCAAAA	GCTACCCAATACCCCTTCCT	45.0	55.0	58.0	58.5	1414-1426 bp	1.0	77.6
ndhF-rpl32_p4	GGAGAAGTCCCACCCCTATT	GCTACCCAATACCCCTTCCT	55.0	55.0	58.1	58.5	987-999 bp	1.0	77.6
ndhF-rpl32_p5	ACTAGGAAAAGCCCACATGC	GCTACCCAATACCCCTTCCT	50.0	55.0	58.2	58.5	1037-1049 bp	1.0	77.0