Hotspot

ndhC-trnV-UAC

LSC · 55809-56418 · 610 bp

Back to Pterocarya

Pi 0.00397

MarkerSeek score 56.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 610
variable_sites: 5
pi: 0.00397
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCCGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	619-756 bp	1.0	81.9
ndhC-trnV-UAC_p2	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	631-768 bp	1.0	80.3
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCCGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	618-755 bp	1.0	79.1
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCCGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	607-744 bp	1.0	78.0
ndhC-trnV-UAC_p5	CCTTTGCTAATCGGGGCTAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.7	57.5	630-767 bp	1.0	77.5