Hotspot

rpoB-trnC-GCA

LSC · 29008-30254 · 1247 bp

Back to Pterocarya

Pi 0.00517

MarkerSeek score 63.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1247
variable_sites: 21
pi: 0.00517
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TACCTCATTTCCACCCCCAA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	1312-1328 bp	1.0	76.9
rpoB-trnC-GCA_p2	TTACCTCATTTCCACCCCCA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	1313-1329 bp	1.0	76.9
rpoB-trnC-GCA_p3	TACCTCATTTCCACCCCCAA	GCGACACCCAGATTTGAACT	50.0	50.0	58.2	58.5	1337-1353 bp	1.0	75.9
rpoB-trnC-GCA_p4	TTACCTCATTTCCACCCCCA	GCGACACCCAGATTTGAACT	50.0	50.0	58.2	58.5	1338-1354 bp	1.0	75.9
rpoB-trnC-GCA_p5	TACCTCATTTCCACCCCCAA	TTTGTTGATCAGGCGACACC	50.0	50.0	58.2	58.8	1349-1365 bp	1.0	73.6