Hotspot

rps2-rpoC2

LSC · 18303-18532 · 230 bp

Back to Pterocarya

Pi 0.0167

MarkerSeek score 66.80

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 230
variable_sites: 10
pi: 0.0167
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCTGCTTCCATCATCTCTTCC	CCCAAACAATCATTGCGAGG	52.4	50.0	58.2	57.7	309-322 bp	1.0	73.6
rps2-rpoC2_p2	ACTCCTGCTTCCATCATCTCT	CCCAAACAATCATTGCGAGG	47.6	50.0	58.2	57.7	312-325 bp	1.0	72.9
rps2-rpoC2_p3	TGACCAAAATGAACTCCTGCT	CCCAAACAATCATTGCGAGG	42.9	50.0	57.8	57.7	324-337 bp	1.0	72.8
rps2-rpoC2_p4	GGTGCCATTCTAGGATTCCA	CCCAAACAATCATTGCGAGG	50.0	50.0	57.0	57.7	360-370 bp	0.9	69.0
rps2-rpoC2_p5	GGTGCCATTCTAGGATTCCA	ACACCCAAACAATCATTGCG	50.0	45.0	57.0	57.6	363-373 bp	0.9	67.8