Hotspot

trnS-GCU-trnG-GCC

LSC · 9456-10264 · 809 bp

Back to Pterocarya

Pi 0.00461

MarkerSeek score 63.90

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 809
variable_sites: 12
pi: 0.00461
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	GGAACGAATCACACTTTTACCA	50.0	40.9	58.0	56.9	629-881 bp	1.0	60.4
trnS-GCU-trnG-GCC_p2	CAATCCGACGCTTTAGTCCA	TGGAACGAATCACACTTTTACC	50.0	40.9	58.0	56.9	630-882 bp	1.0	60.4
trnS-GCU-trnG-GCC_p3	AATCCGACGCTTTAGTCCAC	GGAACGAATCACACTTTTACCA	50.0	40.9	58.0	56.9	628-880 bp	1.0	60.4
trnS-GCU-trnG-GCC_p4	AATCCGACGCTTTAGTCCAC	TGGAACGAATCACACTTTTACC	50.0	40.9	58.0	56.9	629-881 bp	1.0	60.4
trnS-GCU-trnG-GCC_p5	ACAAAAGCGGAAAGAGAGGG	GGAACGAATCACACTTTTACCA	50.0	40.9	58.1	56.9	689-941 bp	1.0	59.6