Hotspot

rpl16

LSC · 85300-86910 · 1611 bp

Back to Pterocarpus

Pi 0.0145

MarkerSeek score 63.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1611
variable_sites: 72
pi: 0.0145
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CCGCTGTTATCGGCTACATT	GGGTAAGCCAGGAAGAATCG	50.0	55.0	58.1	58.0	2307-2370 bp	1.0	84.5
rpl16_p2	CCGCTGTTATCGGCTACATT	GCGGGTACAAAAGGAGTTCA	50.0	50.0	58.1	58.1	2102-2165 bp	1.0	84.4
rpl16_p3	ACCTCGGGTGCTAATGAAAC	GGGTAAGCCAGGAAGAATCG	50.0	55.0	57.9	58.0	2625-2688 bp	1.0	84.0
rpl16_p4	ACCTCGGGTGCTAATGAAAC	GCGGGTACAAAAGGAGTTCA	50.0	50.0	57.9	58.1	2420-2483 bp	1.0	83.8
rpl16_p5	TATCATGCCGTTGCTACGTT	GGGTAAGCCAGGAAGAATCG	45.0	55.0	57.7	58.0	2491-2554 bp	1.0	82.8