Hotspot

rpl32-trnL-UAG

SSC · 117313-118228 · 916 bp

Back to Pterocarpus

Pi 0.026

MarkerSeek score 63.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 916
variable_sites: 59
pi: 0.026
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAGGGGATATTGGACAGCA	GATAAGATGCCATGCCGCTA	50.0	50.0	57.8	57.9	1030-1132 bp	1.0	86.3
rpl32-trnL-UAG_p2	AGAGGGGATATTGGACAGCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.8	57.9	977-1079 bp	1.0	86.3
rpl32-trnL-UAG_p3	AGAGGGGATATTGGACAGCA	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	57.8	58.1	1464-1576 bp	1.0	85.8
rpl32-trnL-UAG_p4	AGAGGGGATATTGGACAGCA	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.8	57.8	970-1072 bp	1.0	85.8
rpl32-trnL-UAG_p5	AGAGGGGATATTGGACAGCA	ATAAGATGCCATGCCGCTAC	50.0	50.0	57.8	58.2	1029-1131 bp	1.0	85.6