Hotspot

rps3-rps19

LSC · 87735-88280 · 546 bp

Back to Pterocarpus

Pi 0.0219

MarkerSeek score 71.70

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 546
variable_sites: 33
pi: 0.0219
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	CAAGTCTGAAACCAAGCGGA	ACTTCCGAGGACATGCAAAA	50.0	45.0	58.4	57.7	617-627 bp	1.0	81.7
rps3-rps19_p2	CAAGTCTGAAACCAAGCGGA	AAACTTCCGAGGACATGCAA	50.0	45.0	58.4	57.7	619-629 bp	1.0	81.6
rps3-rps19_p3	CAAGTCTGAAACCAAGCGGA	AACTTCCGAGGACATGCAAA	50.0	45.0	58.4	57.7	618-628 bp	1.0	81.6
rps3-rps19_p4	AGTCTGAAACCAAGCGGATT	AACTTCCGAGGACATGCAAA	45.0	45.0	57.4	57.7	616-626 bp	1.0	80.4
rps3-rps19_p5	AGTCTGAAACCAAGCGGATT	ACTTCCGAGGACATGCAAAA	45.0	45.0	57.4	57.7	615-625 bp	1.0	80.4