Hotspot

trnQ-UUG-rps16

LSC · 57964-58557 · 594 bp

Back to Pterocarpus

Pi 0.0261
MarkerSeek score 71.40
Primer available yes
Label trnQ-UUG-rps16

Feature Metadata

label_name
trnQ-UUG-rps16
feature_type
igs
region
LSC
length_bp
594
variable_sites
39
pi
0.0261
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-rps16_p1 GAATCAAAAATGGGGCGTGG GTCTTTCAAGTCGCACGTTG 50.0 50.0 58.0 58.0 716-802 bp 1.0 87.7
trnQ-UUG-rps16_p2 GTCGAATCAAAAATGGGGCG GTCTTTCAAGTCGCACGTTG 50.0 50.0 58.1 58.0 719-805 bp 1.0 87.0
trnQ-UUG-rps16_p3 GAATCAAAAATGGGGCGTGG ATGTCTTTCAAGTCGCACGT 50.0 45.0 58.0 58.1 718-804 bp 1.0 86.7
trnQ-UUG-rps16_p4 GAATCAAAAATGGGGCGTGG GCACGTTGCTTTTTACCACA 50.0 45.0 58.0 57.8 704-790 bp 1.0 86.3
trnQ-UUG-rps16_p5 GTCGAATCAAAAATGGGGCG ATGTCTTTCAAGTCGCACGT 50.0 45.0 58.1 58.1 721-807 bp 1.0 86.0