Hotspot

atpI-atpH

LSC · 49089-50432 · 1344 bp

Back to Pterocarpus

Pi 0.0241

MarkerSeek score 73.30

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1344
variable_sites: 84
pi: 0.0241
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TACAAGCGGTATTCAAGCCC	ATAACGGAAGCGGCAGAAAT	50.0	45.0	58.0	58.0	1152-1472 bp	1.0	88.4
atpI-atpH_p2	TACAAGCGGTATTCAAGCCC	CGCAATCCCTTCTACTGCTT	50.0	50.0	58.0	58.0	1240-1560 bp	1.0	88.2
atpI-atpH_p3	TACAAGCGGTATTCAAGCCC	AATAACGGAAGCGGCAGAAA	50.0	45.0	58.0	58.2	1153-1473 bp	1.0	87.2
atpI-atpH_p4	TACAAGCGGTATTCAAGCCC	AGGTCCAATAGAAGCAAGCC	50.0	50.0	58.0	57.6	1192-1512 bp	1.0	85.1
atpI-atpH_p5	TACAAGCGGTATTCAAGCCC	GTCCAATAGAAGCAAGCCCT	50.0	50.0	58.0	57.6	1190-1510 bp	1.0	85.1