Hotspot

psbD-trnT-GGU

LSC · 29443-30916 · 1474 bp

Back to Pterocarpus

Pi 0.013
MarkerSeek score 64.10
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
LSC
length_bp
1474
variable_sites
56
pi
0.013
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 CACAAAAACGAAACGGTCCC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1221-1640 bp 1.0 85.2
psbD-trnT-GGU_p2 CCACAAAAACGAAACGGTCC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1222-1641 bp 1.0 85.2
psbD-trnT-GGU_p3 CCCACAAAAACGAAACGGTC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1223-1642 bp 1.0 85.2
psbD-trnT-GGU_p4 CACAAAAACGAAACGGTCCC TCAGTGGTAGAGTAACGCCA 50.0 50.0 58.2 58.1 1224-1643 bp 1.0 85.0
psbD-trnT-GGU_p5 CCACAAAAACGAAACGGTCC TCAGTGGTAGAGTAACGCCA 50.0 50.0 58.2 58.1 1225-1644 bp 1.0 85.0