Hotspot

trnL-UAA-trnT-UGU

LSC · 14461-15758 · 1298 bp

Back to Pterocarpus

Pi 0.023

MarkerSeek score 67.30

Primer available yes

Label trnL-UAA-trnT-UGU

Feature Metadata

label_name: trnL-UAA-trnT-UGU
feature_type: igs
region: LSC
length_bp: 1298
variable_sites: 61
pi: 0.023
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnT-UGU_p1	TAGCGTCTACCAATTTCGCC	AAGAGAAAAGCCGGCTATCG	50.0	50.0	58.1	58.1	1263-1407 bp	1.0	86.7
trnL-UAA-trnT-UGU_p2	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	50.0	55.0	58.1	57.9	1212-1356 bp	1.0	86.3
trnL-UAA-trnT-UGU_p3	AGTCCGTAGCGTCTACCAAT	AAGAGAAAAGCCGGCTATCG	50.0	50.0	58.2	58.1	1269-1413 bp	1.0	85.3
trnL-UAA-trnT-UGU_p4	TAGCGTCTACCAATTTCGCC	GGAATCGAACCGATGACCAT	50.0	50.0	58.1	57.8	1244-1388 bp	1.0	85.3
trnL-UAA-trnT-UGU_p5	AGTCCGTAGCGTCTACCAAT	GCGATGCTCTAACCTCTGAG	50.0	55.0	58.2	57.9	1218-1362 bp	1.0	84.9