Hotspot

trnK-UUU-rbcL

LSC · 4382-5124 · 743 bp

Back to Pterocarpus

Pi 0.0216

MarkerSeek score 65.10

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 743
variable_sites: 54
pi: 0.0216
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	GAGTTGCCAAGCTCTATCCC	AGTCCTGAATTAGCTGCTGC	55.0	50.0	58.0	58.0	1101-1153 bp	1.0	86.2
trnK-UUU-rbcL_p2	GAGTTGCCAAGCTCTATCCC	AATGAGGGTCGTGATCTTGC	55.0	50.0	58.0	58.0	1161-1213 bp	1.0	86.0
trnK-UUU-rbcL_p3	GGAGTTGCCAAGCTCTATCC	AATGAGGGTCGTGATCTTGC	55.0	50.0	58.0	58.0	1162-1214 bp	1.0	86.0
trnK-UUU-rbcL_p4	GAGTTGCCAAGCTCTATCCC	GCCGTAGCTAATCGAGTAGC	55.0	55.0	58.0	58.0	1206-1258 bp	1.0	85.7
trnK-UUU-rbcL_p5	GGAGTTGCCAAGCTCTATCC	GCCGTAGCTAATCGAGTAGC	55.0	55.0	58.0	58.0	1207-1259 bp	1.0	85.7