Hotspot

trnH-GUG-psbA

LSC · 117-376 · 260 bp

Back to Pterocarpus

Pi 0.0323
MarkerSeek score 68.30
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
260
variable_sites
23
pi
0.0323
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCAGT 55.0 55.0 58.1 57.9 262-336 bp 1.0 88.1
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 265-339 bp 1.0 88.0
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCAGT 50.0 55.0 57.9 57.9 276-350 bp 1.0 87.9
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCAGT 50.0 55.0 57.9 57.9 275-349 bp 1.0 87.9
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCAGT 50.0 55.0 57.9 57.9 277-351 bp 1.0 87.8