Hotspot

petB

LSC · 75705-77101 · 1397 bp

Back to Pternopetalum

Pi 0.0122

MarkerSeek score 61.10

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1397
variable_sites: 17
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	CTATTTTGGCAGTTCGACCG	CCCCCAGTGATAAAGGATGG	50.0	55.0	57.2	57.3	1495-1496 bp	1.0	69.1
petB_p2	AGCCCATTCTATTTTGGCAGT	CCCCCAGTGATAAAGGATGG	42.9	55.0	57.9	57.3	1503-1504 bp	1.0	66.7
petB_p3	TATTTTGGCAGTTCGACCGC	CCCCCAGTGATAAAGGATGG	50.0	55.0	59.2	57.3	1494-1495 bp	1.0	66.0
petB_p4	GCCCATTCTATTTTGGCAGT	CCCCCAGTGATAAAGGATGG	45.0	55.0	56.4	57.3	1502-1503 bp	1.0	62.4
petB_p5	TCTATTTTGGCAGTTCGACCG	CCCCCAGTGATAAAGGATGG	47.6	55.0	58.7	57.3	1496-1497 bp	1.0	62.3