Hotspot

rps12-rps7

IRa · 142764-142818 · 55 bp

Back to Pternopetalum

Pi 0.0182

MarkerSeek score 63.00

Primer available yes

Label rps12-rps7

Feature Metadata

label_name: rps12-rps7
feature_type: igs
region: IRa
length_bp: 55
variable_sites: 1
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rps7_p1	GGCTTGGAGGGAGATCTTTC	TTCTTCTGCAGTACCTCGAC	55.0	50.0	57.7	57.0	151 bp	1.0	69.7
rps12-rps7_p2	GGCTTGGAGGGAGATCTTTC	TCTTCTGCAGTACCTCGACG	55.0	55.0	57.7	59.2	150 bp	1.0	68.2
rps12-rps7_p3	TATGTACGGCTTGGAGGGAG	TTCTTCTGCAGTACCTCGAC	55.0	50.0	58.6	57.0	158 bp	1.0	67.7
rps12-rps7_p4	GGCTTGGAGGGAGATCTTTC	TTTCTTCTGCAGTACCTCGAC	55.0	47.6	57.7	57.7	152 bp	1.0	67.4
rps12-rps7_p5	GGCTTGGAGGGAGATCTTTC	CTTCTGCAGTACCTCGACG	55.0	57.9	57.7	57.7	149 bp	1.0	67.3