Hotspot

ndhG-ndhI

SSC · 120766-121114 · 349 bp

Back to Pternopetalum

Pi 0.0264

MarkerSeek score 57.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 349
variable_sites: 9
pi: 0.0264
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAGACCCGATCCCAGAAAGA	TGGTCAACAAACCGTACGAG	50.0	50.0	58.0	58.1	872-878 bp	1.0	80.6
ndhG-ndhI_p2	AAACCATCGACGTAACTCCG	TGGTCAACAAACCGTACGAG	50.0	50.0	58.0	58.1	1125-1131 bp	1.0	80.4
ndhG-ndhI_p3	GCAATCGCACCTATTAACGC	TGGTCAACAAACCGTACGAG	50.0	50.0	58.0	58.1	1330-1336 bp	1.0	80.2
ndhG-ndhI_p4	AAACCATCGACGTAACTCCG	GTACATCGGCCAAGGTTTCA	50.0	50.0	58.0	58.2	1098-1104 bp	1.0	80.1
ndhG-ndhI_p5	GCAATCGCACCTATTAACGC	GTACATCGGCCAAGGTTTCA	50.0	50.0	58.0	58.2	1303-1309 bp	1.0	79.8