Hotspot

trnL-UAG-ccsA

SSC · 116282-116383 · 102 bp

Back to Pternopetalum

Pi 0.0588

MarkerSeek score 75.90

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 102
variable_sites: 6
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	ACAATGGAAACGATCGAAAAGG	50.0	40.9	58.0	57.6	235 bp	1.0	62.6
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	ACAATGGAAACGATCGAAAAGG	50.0	40.9	58.0	57.6	234 bp	1.0	62.6
trnL-UAG-ccsA_p3	CTTAGGAAGCAGTGCGAGAG	ACAATGGAAACGATCGAAAAGG	55.0	40.9	58.1	57.6	212 bp	1.0	62.3
trnL-UAG-ccsA_p4	CTCTTAGGAAGCAGTGCGAG	ACAATGGAAACGATCGAAAAGG	55.0	40.9	58.1	57.6	214 bp	1.0	62.2
trnL-UAG-ccsA_p5	TGAATTCGAACAAGCCGCTA	ACAATGGAAACGATCGAAAAGG	45.0	40.9	57.9	57.6	257 bp	1.0	61.8