Hotspot

ndhF-rpl32

SSC · 114023-115107 · 1085 bp

Back to Pternopetalum

Pi 0.0213

MarkerSeek score 67.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1085
variable_sites: 23
pi: 0.0213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGACGGAAAAAGAAG	GCTGCCCAATATCCCTTACC	50.0	55.0	58.0	58.1	1266-1348 bp	1.0	80.7
ndhF-rpl32_p2	GTTGCCGACGGAAAAAGAAG	GGCTGCCCAATATCCCTTAC	50.0	55.0	58.0	58.1	1267-1349 bp	1.0	80.7
ndhF-rpl32_p3	TGCCGACGGAAAAAGAAGAA	GCTGCCCAATATCCCTTACC	45.0	55.0	58.0	58.1	1264-1346 bp	1.0	80.7
ndhF-rpl32_p4	TGCCGACGGAAAAAGAAGAA	GGCTGCCCAATATCCCTTAC	45.0	55.0	58.0	58.1	1265-1347 bp	1.0	80.7
ndhF-rpl32_p5	TTGCCGACGGAAAAAGAAGA	GCTGCCCAATATCCCTTACC	45.0	55.0	58.0	58.1	1265-1347 bp	1.0	80.7