Hotspot

rps7-rps12

IRb · 97666-97720 · 55 bp

Back to Pternopetalum

Pi 0.0182

MarkerSeek score 63.00

Primer available yes

Label rps7-rps12

Feature Metadata

label_name: rps7-rps12
feature_type: igs
region: IRb
length_bp: 55
variable_sites: 1
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-rps12_p1	TTCTTCTGCAGTACCTCGAC	GGCTTGGAGGGAGATCTTTC	50.0	55.0	57.0	57.7	151 bp	1.0	69.7
rps7-rps12_p2	TCTTCTGCAGTACCTCGACG	GGCTTGGAGGGAGATCTTTC	55.0	55.0	59.2	57.7	150 bp	1.0	68.2
rps7-rps12_p3	TTCTTCTGCAGTACCTCGAC	TATGTACGGCTTGGAGGGAG	50.0	55.0	57.0	58.6	158 bp	1.0	67.7
rps7-rps12_p4	TTTCTTCTGCAGTACCTCGAC	GGCTTGGAGGGAGATCTTTC	47.6	55.0	57.7	57.7	152 bp	1.0	67.4
rps7-rps12_p5	CTTCTGCAGTACCTCGACG	GGCTTGGAGGGAGATCTTTC	57.9	55.0	57.7	57.7	149 bp	1.0	67.3