Hotspot

ycf4-cemA

LSC · 61249-61934 · 686 bp

Back to Pternopetalum

Pi 0.0148

MarkerSeek score 68.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 686
variable_sites: 10
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CGAACAAAAAGCTGCCGAAT	TCCACCAGGGCAAAAATACT	45.0	45.0	57.9	57.0	802-811 bp	1.0	73.6
ycf4-cemA_p2	AATCGAACAAAAAGCTGCCG	TCCACCAGGGCAAAAATACT	45.0	45.0	57.9	57.0	805-814 bp	1.0	73.5
ycf4-cemA_p3	CATTCCCTTGACTCGTACGG	TCCACCAGGGCAAAAATACT	55.0	45.0	58.1	57.0	847-856 bp	1.0	73.2
ycf4-cemA_p4	CCATTCCCTTGACTCGTACG	TCCACCAGGGCAAAAATACT	55.0	45.0	58.1	57.0	848-857 bp	1.0	73.2
ycf4-cemA_p5	CGAACAAAAAGCTGCCGAAT	GAGATAAATCCACCAGGGCA	45.0	50.0	57.9	57.0	810-819 bp	1.0	73.2