Hotspot

trnE-UUC-trnT-GGU

LSC · 31340-32074 · 735 bp

Back to Pternopetalum

Pi 0.0195

MarkerSeek score 63.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 14
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	822-839 bp	1.0	81.6
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	787-804 bp	1.0	80.9
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	819-836 bp	1.0	80.4
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	AGCTAAAGCCCCTTATCGGA	50.0	50.0	57.8	58.2	845-862 bp	1.0	80.0
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	TTGAACCGATGACTTACGCC	50.0	50.0	57.8	58.3	824-841 bp	1.0	79.4