Hotspot

trnD-GUC-trnY-GUA

LSC · 30986-31106 · 121 bp

Back to Pternopetalum

Pi 0.0354

MarkerSeek score 69.10

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 121
variable_sites: 4
pi: 0.0354
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	183-191 bp	1.0	79.4
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	182-190 bp	1.0	79.4
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	204-212 bp	1.0	78.0
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	203-211 bp	1.0	77.8
trnD-GUC-trnY-GUA_p5	TTGAATCTGTCGGGACTGAC	TGGCAATATGTCTACGCTGG	50.0	50.0	57.3	57.8	237-245 bp	1.0	76.1