Hotspot

petN-psbM

LSC · 29061-30157 · 1097 bp

Back to Pternopetalum

Pi 0.0202

MarkerSeek score 60.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1097
variable_sites: 22
pi: 0.0202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	GCTTTTCTGCTTATCATTTATGT	50.0	30.4	57.6	53.7	1179-1189 bp	1.0	42.0
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	GCTTTTCTGCTTATCATTTATGT	50.0	30.4	58.2	53.7	1240-1250 bp	1.0	41.9
petN-psbM_p3	ATAGTAAGTCTCGCTTGGGC	GCTTTTCTGCTTATCATTTATGT	50.0	30.4	57.1	53.7	1241-1251 bp	1.0	41.9
petN-psbM_p4	CTCGCTTGGGCTGCTTTAAT	GCTTTTCTGCTTATCATTTATGT	50.0	30.4	58.9	53.7	1232-1242 bp	1.0	41.9
petN-psbM_p5	TGGGCTGCTTTAATGGTAGTC	GCTTTTCTGCTTATCATTTATGT	47.6	30.4	58.0	53.7	1226-1236 bp	1.0	41.9