Hotspot

rpoB-trnC-GCA

LSC · 27000-28207 · 1208 bp

Back to Pternopetalum

Pi 0.0182

MarkerSeek score 64.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1208
variable_sites: 22
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATCTCGGA	CTTACCACTCGGCCATATCG	50.0	55.0	57.8	57.9	1254-1266 bp	1.0	79.5
rpoB-trnC-GCA_p2	TCCCTCATTTCCATCTCGGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1294-1306 bp	1.0	79.3
rpoB-trnC-GCA_p3	AGCCCTGATCCATGAACCTA	CTTACCACTCGGCCATATCG	50.0	55.0	57.8	57.9	1321-1333 bp	1.0	79.2
rpoB-trnC-GCA_p4	AGCCCTGATCCATGAACCTA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1361-1373 bp	1.0	79.1
rpoB-trnC-GCA_p5	GCCCTGATCCATGAACCTAC	CTTACCACTCGGCCATATCG	55.0	55.0	57.7	57.9	1320-1332 bp	1.0	78.7