Hotspot

rps16-trnQ-UUG

LSC · 5687-7091 · 1405 bp

Back to Pternopetalum

Pi 0.0214
MarkerSeek score 61.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1405
variable_sites
30
pi
0.0214
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TGCTTTCTACCACAGCGTTT AAATATAGATGGGGCGTGGC 45.0 50.0 58.0 57.8 1524-1547 bp 1.0 79.7
rps16-trnQ-UUG_p2 TTGCTTTCTACCACAGCGTT AAATATAGATGGGGCGTGGC 45.0 50.0 58.0 57.8 1525-1548 bp 1.0 79.7
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA AAATATAGATGGGGCGTGGC 45.0 50.0 58.1 57.8 1535-1558 bp 1.0 78.8
rps16-trnQ-UUG_p4 CGGATCGTGTCCTTCAAGTC AAATATAGATGGGGCGTGGC 55.0 50.0 58.4 57.8 1550-1573 bp 1.0 77.0
rps16-trnQ-UUG_p5 GGATCGTGTCCTTCAAGTCG AAATATAGATGGGGCGTGGC 55.0 50.0 58.4 57.8 1549-1572 bp 1.0 77.0