Hotspot

ccsA

SSC · 117341-118303 · 963 bp

Back to Pternandra

Pi 0.0114

MarkerSeek score 51.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 963
variable_sites: 11
pi: 0.0114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CCCCATCGAGCTACTTAGGA	GTGGAATGGCGATTCTCCTT	55.0	50.0	58.0	58.0	2065-2068 bp	1.0	81.2
ccsA_p2	TCCCCATCGAGCTACTTAGG	GTGGAATGGCGATTCTCCTT	55.0	50.0	58.0	58.0	2066-2069 bp	1.0	81.2
ccsA_p3	CCCCATCGAGCTACTTAGGA	TCAATGGCTTCCCTTGCATT	55.0	45.0	58.0	58.1	2015-2018 bp	1.0	81.1
ccsA_p4	GTGAAATCGGTAGACACGCT	GTGGAATGGCGATTCTCCTT	50.0	50.0	58.0	58.0	1841-1845 bp	1.0	80.8
ccsA_p5	TGAAATCGGTAGACACGCTG	GTGGAATGGCGATTCTCCTT	50.0	50.0	58.0	58.0	1840-1844 bp	1.0	80.8