Hotspot

rps15-ycf1

SSC · 126539-127023 · 485 bp

Back to Pternandra

Pi 0.025

MarkerSeek score 57.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 485
variable_sites: 12
pi: 0.025
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGCAGTCGTTGTCGTTTTC	TCCACTAAACGGCGTAGAGA	50.0	50.0	58.0	58.2	965-968 bp	1.0	80.6
rps15-ycf1_p2	CAGCAGTCGTTGTCGTTTTC	TCTCCACTAAACGGCGTAGA	50.0	50.0	58.0	58.2	967-970 bp	1.0	80.6
rps15-ycf1_p3	TTTTACGTAGACCTCGCTGC	TCCACTAAACGGCGTAGAGA	50.0	50.0	58.0	58.2	939-942 bp	1.0	80.5
rps15-ycf1_p4	TTTTACGTAGACCTCGCTGC	TCTCCACTAAACGGCGTAGA	50.0	50.0	58.0	58.2	941-944 bp	1.0	80.5
rps15-ycf1_p5	CAGCAGTCGTTGTCGTTTTC	CTCCACTAAACGGCGTAGAG	50.0	55.0	58.0	57.8	966-969 bp	1.0	80.5