Hotspot

rpl32-trnL-UAG

SSC · 116860-117155 · 296 bp

Back to Pternandra

Pi 0.0441

MarkerSeek score 72.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 296
variable_sites: 13
pi: 0.0441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCGTTGAAGGCTTTTTCGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	397 bp	1.0	81.4
rpl32-trnL-UAG_p2	GCGTTGAAGGCTTTTTCGTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	398 bp	1.0	81.4
rpl32-trnL-UAG_p3	GCGTTGAAGGCTTTTTCGTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	407 bp	1.0	80.4
rpl32-trnL-UAG_p4	TATTTTGCAGCGTTGAAGGC	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.7	58.0	406 bp	1.0	79.9
rpl32-trnL-UAG_p5	TATTTTGCAGCGTTGAAGGC	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.7	58.0	407 bp	1.0	79.9