Hotspot

petA-psbJ

LSC · 64602-65482 · 881 bp

Back to Pternandra

Pi 0.0178

MarkerSeek score 70.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 881
variable_sites: 13
pi: 0.0178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCCATTACGTGTCCAAGGTC	TATGGTTCATACTCCGGCCT	50.0	50.0	57.2	57.9	886-1035 bp	1.0	78.3
petA-psbJ_p2	CCATTACGTGTCCAAGGTCT	TATGGTTCATACTCCGGCCT	50.0	50.0	57.2	57.9	885-1034 bp	1.0	78.2
petA-psbJ_p3	CCATTACGTGTCCAAGGTCTT	TATGGTTCATACTCCGGCCT	47.6	50.0	57.9	57.9	885-1034 bp	1.0	75.9
petA-psbJ_p4	TCCATTACGTGTCCAAGGTC	ACCGGTATTCTTGTGATCGG	50.0	50.0	57.2	57.4	925-1074 bp	1.0	74.2
petA-psbJ_p5	TCCATTACGTGTCCAAGGTC	CCGGTATTCTTGTGATCGGT	50.0	50.0	57.2	57.4	924-1073 bp	1.0	74.2