Hotspot

ndhK-ndhC

LSC · 52089-52152 · 64 bp

Back to Pternandra

Pi 0.0469

MarkerSeek score 69.60

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 3
pi: 0.0469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	TTTGGGTTGTTCGAGCAAGT	CATGGCGAAAAGGAGCATTG	45.0	50.0	58.2	58.1	139-140 bp	1.0	79.1
ndhK-ndhC_p2	TTGGGTTGTTCGAGCAAGTA	CATGGCGAAAAGGAGCATTG	45.0	50.0	57.4	58.1	138-139 bp	1.0	76.1
ndhK-ndhC_p3	TGGGTTGTTCGAGCAAGTAT	CATGGCGAAAAGGAGCATTG	45.0	50.0	57.1	58.1	137-138 bp	1.0	74.3
ndhK-ndhC_p4	TTTTGGGTTGTTCGAGCAAG	CATGGCGAAAAGGAGCATTG	45.0	50.0	57.1	58.1	140-141 bp	1.0	74.2
ndhK-ndhC_p5	TTTGGGTTGTTCGAGCAAGTA	CATGGCGAAAAGGAGCATTG	42.9	50.0	58.1	58.1	139-140 bp	1.0	72.4