Hotspot

trnD-GUC-trnY-GUA

LSC · 31119-31547 · 429 bp

Back to Pternandra

Pi 0.026

MarkerSeek score 62.20

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 429
variable_sites: 11
pi: 0.026
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GCGGTGCTCTAACCAATTGA	ATGGATTCTTCCTGGGTCGA	50.0	50.0	58.6	58.1	551-557 bp	1.0	77.1
trnD-GUC-trnY-GUA_p2	GCGGTGCTCTAACCAATTGA	TGGATTCTTCCTGGGTCGAT	50.0	50.0	58.6	58.1	550-556 bp	1.0	77.1
trnD-GUC-trnY-GUA_p3	GCGGTGCTCTAACCAATTGA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.6	57.8	494-500 bp	1.0	76.4
trnD-GUC-trnY-GUA_p4	GCGGTGCTCTAACCAATTGA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.6	57.8	493-499 bp	1.0	76.4
trnD-GUC-trnY-GUA_p5	GCGGTGCTCTAACCAATTGA	GGATTCTTCCTGGGTCGATG	50.0	55.0	58.6	57.8	549-555 bp	1.0	76.1