Hotspot

trnH-GUG-psbA

LSC · 76-463 · 388 bp

Back to Pternandra

Pi 0.0525

MarkerSeek score 75.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 388
variable_sites: 20
pi: 0.0525
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	453-455 bp	1.0	83.2
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	456-458 bp	1.0	83.1
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	467-469 bp	1.0	83.0
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	466-468 bp	1.0	83.0
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	468-470 bp	1.0	83.0