Hotspot

rpl16

LSC · 77003-78097 · 1095 bp

Back to Pteris

Pi 0.0956
MarkerSeek score 84.60
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1095
variable_sites
306
pi
0.0956
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 CGCTGTTGTCTGCTACATCT CACCGATAAAGGCGTCAAGA 50.0 50.0 58.0 58.0 1682-1703 bp 0.857143 83.9
rpl16_p2 CGCTGTTGTCTGCTACATCT TGCCTTTCCGACGAACTATG 50.0 50.0 58.0 58.0 1731-1752 bp 0.857143 83.8
rpl16_p3 CGCTGTTGTCTGCTACATCT GTGCCTTTCCGACGAACTAT 50.0 50.0 58.0 58.0 1732-1753 bp 0.857143 83.8
rpl16_p4 CGGTTGCCTGTTCCTAGTAC CACCGATAAAGGCGTCAAGA 55.0 50.0 58.0 58.0 1728-1749 bp 0.857143 83.7
rpl16_p5 CGGTTGCCTGTTCCTAGTAC TGCCTTTCCGACGAACTATG 55.0 50.0 58.0 58.0 1777-1798 bp 0.857143 83.6