Hotspot

petB

LSC · 70614-72082 · 1469 bp

Back to Pteris

Pi 0.0841
MarkerSeek score 84.40
Primer available yes
Label petB

Feature Metadata

label_name
petB
feature_type
gene
region
LSC
length_bp
1469
variable_sites
365
pi
0.0841
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB_p1 GGCATAACCGGTGAAGCTAA GTTTATTTACCCCGACGGCT 50.0 50.0 58.0 58.0 2538-2570 bp 0.714286 76.4
petB_p2 TCGTCGCAATTAATCCGGTT GCGGCAACAGAAGAACAATC 45.0 50.0 58.0 58.0 1618-1635 bp 0.571429 71.2
petB_p3 TTCGTCGCAATTAATCCGGT GCGGCAACAGAAGAACAATC 45.0 50.0 58.0 58.0 1619-1636 bp 0.571429 71.2
petB_p4 TCGTCGCAATTAATCCGGTT GTTTATTTACCCCGACGGCT 45.0 50.0 58.0 58.0 2033-2061 bp 0.571429 69.7
petB_p5 TTCGTCGCAATTAATCCGGT GTTTATTTACCCCGACGGCT 45.0 50.0 58.0 58.0 2034-2062 bp 0.571429 69.7