Hotspot

atpB-rbcL

LSC · 51214-51858 · 645 bp

Back to Pteris

Pi 0.102
MarkerSeek score 84.90
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
645
variable_sites
199
pi
0.102
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CCACATCCAAAACTGGTCCA CCAGTAGGGGACGTCCATAT 50.0 55.0 58.0 58.0 1216-1250 bp 0.857143 82.0
atpB-rbcL_p2 CGGGTTCGCCAAGTACATTA CCAGTAGGGGACGTCCATAT 50.0 55.0 58.0 58.0 1468-1502 bp 0.857143 81.3
atpB-rbcL_p3 AGAGTGCTTTCACCAACAGG CCAGTAGGGGACGTCCATAT 50.0 55.0 58.0 58.0 1436-1470 bp 0.857143 81.3
atpB-rbcL_p4 CCACATCCAAAACTGGTCCA CCACCACGAAGGCATTCATA 50.0 50.0 58.0 58.0 1292-1326 bp 0.857143 81.3
atpB-rbcL_p5 GAGTGCTTTCACCAACAGGA CCAGTAGGGGACGTCCATAT 50.0 55.0 58.0 58.0 1435-1469 bp 0.857143 81.3