Hotspot

rpoB-trnD-GUC

LSC · 26387-27165 · 779 bp

Back to Pteris

Pi 0.153
MarkerSeek score 84.50
Primer available yes
Label rpoB-trnD-GUC

Feature Metadata

label_name
rpoB-trnD-GUC
feature_type
igs
region
LSC
length_bp
779
variable_sites
341
pi
0.153
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnD-GUC_p1 TCTCCGACTGAAGGTTCTGT TCTACCTTCTCCACGATCCC 50.0 55.0 58.0 57.9 1318-1330 bp 0.571429 72.2
rpoB-trnD-GUC_p2 CTGGTACATATGGATCGGCG TCTACCTTCTCCACGATCCC 55.0 55.0 58.0 57.9 1371-1383 bp 0.571429 71.8
rpoB-trnD-GUC_p3 TCTCCGACTGAAGGTTCTGT GGGGGTATTCAAGCTCCAAG 50.0 55.0 58.0 57.9 1379 bp 0.142857 45.2
rpoB-trnD-GUC_p4 CTGGTACATATGGATCGGCG GGGGGTATTCAAGCTCCAAG 55.0 55.0 58.0 57.9 1432 bp 0.142857 45.2
rpoB-trnD-GUC_p5 GCATCCTCCGGTGATTCAAT GGGGGTATTCAAGCTCCAAG 50.0 55.0 58.0 57.9 1844 bp 0.142857 45.0