Hotspot

matK-rps16

LSC · 2861-3531 · 671 bp

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Pi 0.16
MarkerSeek score 84.50
Primer available yes
Label matK-rps16

Feature Metadata

label_name
matK-rps16
feature_type
igs
region
LSC
length_bp
671
variable_sites
294
pi
0.16
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-rps16_p1 GTTTCAGCCCCTCTCTGATG AACTCGACGAAGGGTAAAGC 55.0 50.0 58.0 57.9 1402-1434 bp 0.571429 72.0
matK-rps16_p2 ATGTCTGGTCCGTTTGATCG AACTCGACGAAGGGTAAAGC 50.0 50.0 58.0 57.9 1233-1236 bp 0.571429 71.3
matK-rps16_p3 ATGTCTGGTCCGTTTGATCG AAGGGTAAAGCATCCGTGTC 50.0 50.0 58.0 57.9 1224-1227 bp 0.571429 71.1
matK-rps16_p4 ATGTCTGGTCCGTTTGATCG GAAGGGTAAAGCATCCGTGT 50.0 50.0 58.0 57.9 1225-1228 bp 0.571429 71.1
matK-rps16_p5 TGGTCCGTTTGATCGTTGTT AACTCGACGAAGGGTAAAGC 45.0 50.0 58.0 57.9 1228-1231 bp 0.571429 71.1