Hotspot

ycf1

Genome · 121260-126323 · 5064 bp

Back to Pteridium

Pi 0.00454

MarkerSeek score 48.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5064
variable_sites: 23
pi: 0.00454
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CAAACGATGAGCCGAAACAA	ACGAAAGAGAAGAAGCGGAC	45.0	50.0	57.3	57.9	230 bp	1.0	75.1
ycf1_p2	CAAACGATGAGCCGAAACAA	AAGACCTACGGCCTAACACT	45.0	50.0	57.3	58.1	306 bp	1.0	74.8
ycf1_p3	CAAACGATGAGCCGAAACAA	AGTCGCAGATTTCAACGTGT	45.0	45.0	57.3	58.1	279 bp	1.0	74.4
ycf1_p4	GATGAGCCGAAACAATGCAT	ACGAAAGAGAAGAAGCGGAC	45.0	50.0	57.1	57.9	225 bp	1.0	73.7
ycf1_p5	GATGAGCCGAAACAATGCAT	AAGACCTACGGCCTAACACT	45.0	50.0	57.1	58.1	301 bp	1.0	73.4