Hotspot

rps8

Genome · 77962-78360 · 399 bp

Back to Pteridium

Pi 0.01

MarkerSeek score 56.70

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: Genome
length_bp: 399
variable_sites: 4
pi: 0.01
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	ACCTCCCAGATTATTCACAGA	GAGGTTGGGAATCATTCTGGT	42.9	47.6	55.8	57.6	566-568 bp	1.0	43.6
rps8_p2	AACCTCCCAGATTATTCACAGA	GAGGTTGGGAATCATTCTGGT	40.9	47.6	56.5	57.6	567-569 bp	1.0	41.8
rps8_p3	ACCTCCCAGATTATTCACAGA	AGGTTGGGAATCATTCTGGT	42.9	45.0	55.8	56.4	565-567 bp	1.0	41.8
rps8_p4	ACCTCCCAGATTATTCACAGA	AGAGGTTGGGAATCATTCTGG	42.9	47.6	55.8	57.3	567-569 bp	1.0	41.3
rps8_p5	ACCTCCCAGATTATTCACAGAT	GAGGTTGGGAATCATTCTGGT	40.9	47.6	56.3	57.6	566-568 bp	1.0	40.6