Hotspot

petD

Genome · 74383-75503 · 1121 bp

Back to Pteridium

Pi 0.00357

MarkerSeek score 60.80

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: Genome
length_bp: 1121
variable_sites: 4
pi: 0.00357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	GTTATCCGGCGGATTGAGTT	GGAAGTCTTGCTCCATGGAG	50.0	55.0	58.1	58.0	1273 bp	1.0	79.6
petD_p2	TCCGGCGGATTGAGTTATTG	GGAAGTCTTGCTCCATGGAG	50.0	55.0	58.1	58.0	1269 bp	1.0	79.6
petD_p3	AAGTTATCCGGCGGATTGAG	GGAAGTCTTGCTCCATGGAG	50.0	55.0	57.8	58.0	1275 bp	1.0	78.0
petD_p4	CCGGCGGATTGAGTTATTGT	GGAAGTCTTGCTCCATGGAG	50.0	55.0	58.3	58.0	1268 bp	1.0	77.3
petD_p5	GTTATCCGGCGGATTGAGTT	TCTAGGGAAGTCTTGCTCCA	50.0	50.0	58.1	57.4	1278 bp	1.0	74.9